No abnormal behavior, clinical signs of chronic toxicity or inflammation were observed. BC attenuated CP-induced genotoxic and myelotoxic effects.Ī hydrogel of bacterial cellulose of 0.8%, biopolymer produced from sugarcane molasses and synthesized from a bacteria called Zoogloea sp., was analyzed for its composition and tested by two routes of administration, subcutaneous and intraperitoneal, to clarify the local and systemic adverse effects after skin implantation in Wistar rats (24 males, 24 females, 55 days old). BC was not cytotoxic, genotoxic or acutely toxic. There was no alteration in the LDH release in the wells where C3A cells were treated with increasing concentrations of BC compared to the wells where the cells received the cell culture medium only (background of approximately 20% cell death), indicated that in the dose range tested BC was not cytotoxic. BC attenuated CP-induced and inhibition the incidence of MNPCE (female: 46.94% male: 22.7%) and increased the ratio of PCE/NCE (female: 46.10% male: 35.25%). The treatment with the BC in a single oral dose (2000 mg/kg body weight) caused no deaths or signs of toxicity. BC (0.33-170 μg/mL) added to C3A cell culture medium caused no elevation in LDH release over the background level recorded in untreated cell wells. The genotoxicity of BC was evaluated in vivo by the micronucleus assay. Acute toxicity was tested in adults Wistar rats treated with a single dose of BC. Cytotoxicity of BC was evaluated in cultured C3A hepatoma cells (HepG2/C3A) using a lactate dehydrogenase (LDH) activity assay. The acute toxicity, cytotoxicity, genotoxicity and antigenotoxic effects of BC were studied.
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